Immune Repertoire

Immune receptor repertoire sequencing

In recent years, high-throughput immune repertoire sequencing has led to unprecedented quantitative insight into lymphocyte diversity and adaptive immunity, expanding our understanding of key processes in systems immunology. We have developed DeepImmune sequencing for ultra-deep investigation of immune repertoires of human B cells and T cells, and PairedImmune sequencing for paired repertoire analysis from human B cells and identification of high-affinity monoclonal antibodies.

DeepImmune sequencing – In-depth analysis of B cell and T cell receptor repertoires

  • Sequencing of antibody heavy chains and T cell receptor beta chains
  • Use of normalized primer sets combined with molecular identifiers for unbiased quantitative determination of B cell and T cell receptor copy numbers
  • Results in ultra-deep characterization of immune receptor repertoires
  • Sensitive detection of differences among samples and sample cohorts

Application areas

  • Identification of induced/decreased antibodies and T cell receptors
  • Identification of disease markers
  • Identification of tumor-infiltrating lymphocytes (TILs)

PairedImmune sequencing – Obtaining high-affinity monoclonal antibodies

  • Paired sequencing of antibody heavy and light chains from B cell populations
  • Use of our patented novel technology to retain the endogenous pairing of the antibody heavy and light chain from each individual B cell
  • Results in thousands of paired heavy chain-light chain clonotypes per sample
  • Clonable antibody sequences for production of high-affinity mAbs

Application areas

Identification of high-affinity monoclonal antibodies for production and application in diagnostic and therapeutic settings

Current developments

Adaptation of the PairedImmune method for application to T cell receptor paired alpha and beta chains from T cell populations