Service portfolio

The Alacris team has long-standing expertise in next generation sequencing technologies, large-scale operations, data management, high-dimensional data analytics and bioinformatics systems, allowing us to offer a wide range of cutting-edge services. Our staff is dedicated to excellence, providing quality-certified, personalized services to our customers.

Certified NGS Services

Our customers appreciate the closed, confidential and secure IT infrastructure with ISO9001, Illumina CSPro and Agilent Service Provider certified sequencing pipeline. We are operating all NGS procedures according to strict quality control standards.

Our NGS activities are implemented in a number of research projects and translational activities. We own several patent applications, one of which was licensed by NGS market leaders.

Our NGS service list:

  • Whole genome sequencing (WGS)
  • Shallow WGS for CNV analysis
  • Whole exome sequencing (WES)
  • Targeted sequencing
  • Strand-specific RNA sequencing (mRNA and ribo-depleted)
  • Small RNA /microRNA sequencing
  • ChIP-Seq (Chromatin Immuno Precipitation DNA-Sequencing)
  • MeDIP-Seq (Methylated DNA  Immuno Precipitation Sequencing)
  • Immune repertoire sequencing

Focus on High Resolution Transcriptomics

Alacris Theranostics staff members are inventors of a strand-specific RNAseq protocol (Parkhomchuk et al. 2009, Sultan et al. 2012), used by market leaders in the NGS field. We were first to publish the benefits of RNAseq as compared to microarrays (Sultan et al. 2008). We know what matters.

Strand-specific high resolution transcriptomics provides an essential data layer for:

  • Identifying cancer driving fusion genes
  • Quantitative gene expression
  • Validating variants indentified by WGS/WES
  • Gene variant allele-specific expression
  • Detecting long non-coding RNAs
  • Identifying differentially spliced transcripts isoforms
  • Identifying novel transcription units
  • Analyzing eRNAs
  • Analyzing eQTLs
  • Identifying specific signatures and biomarkers

NGS-Associated Services

Project planning:

  • Selection of the optimal cost-efficient sequencing strategy
  • Assistance in experimental design

Sample processing:

  • Isolation of nucleic acids from different samples (cell cultures, frozen tissue, blood, FFPE, etc.)
  • DNA and RNA quantification and quality control

Sequencing libraries:

  • Standard and project-specific library preparation
  • Protocols with built-in QC steps
  • Quality check of libraries

NGSight & Oncovar: Integrated Genomic/Transcriptomic Analysis

Alacris Theranostics offers one of the most comprehensive NGS analysis pipelines available on the market today.

NGSightTM: we have developed a highly efficient analysis pipeline for NGS data that can be tailored to the needs of each project. The use of multiple mapping algorithms, project-specific data pre-processing, output filtering and data integration make NGSightTM  a powerful and flexible analysis system for all NGS applications.

OncovarTM is the specialized NGSightTM analysis pipeline developed by cancer research experts at Alacris. OncovarTM extracts the most relevant information for the therapy decision process.

CMTA: Comprehensive Molecular Tumor Analysis

A unique personalized solution for cancer patients

Cancer is a complex and heterogeneous disease. Every tumor is unique and demands individual molecular analyses to comprehend its complexity. We offer a highly comprehensive integrated NGS genomic and transcriptomic analysis of tumors that fulfils the highest demands for quality. CMTA is one of the most comprehensive precision medicine product(s) on the oncology market today.

The strength of the CMTA is the use of quantitative high-resolution transcriptome data, enhancing the quality and value of the molecular profiling.

Based on our unique OncovarTM pipeline specially developed for NGS-based molecular oncology, the CMTA provides a personalized approach to cancer management and beyond, delivering an ultra-deep, fully annotated, analysis of the patient’s tumor and reference genome beyond the state of the art.

Biomarker identification

Alacris has established and developed methodologies for complex (omics-driven) biomarker identification that allows (i) the selection of patients or adequate biomarker sets to stratify patients for targeted clinical trials and (ii) biomarker identification as companion diagnostics for personalized oncology. We carry out statistical analyses of genome and other omics-data for the identification of predictive and prognostic biomarkers. The methods we apply for the identification of biomarkers range from basic statistical tests (e.g. Mann-Whitney-U-test) to more sophisticated classification algorithms (e.g. logistic regression, support vector machines, random forests). We successfully applied our biomarker analysis expertise in the framework of the IMI Oncotrack project, leading to novel predictive signatures of cetuximab and 5-FU response in colon cancer (Schuette et al. 2017).

In clinical trial scenarios, the patients that are most likely to benefit from a particular drug/drug combination can be selected based on biomarkers identified, for inclusion in smaller and less expensive clinical trials.

ModCell

Our proprietary ModCellTM technology is a new, revolutionary systems medicine modeling tool for optimizing predictive personalized medicine and drug development.  A simulation tool that provides a gateway to the complex molecular processes occurring within each of our cells, fostering insight and discovery for clinical application and in silico testing of therapy response.

Virtual clinical trials

In ‘virtual clinical trials’ ModCellTM facilitates applications for the pharmaceutical/biotechnology industry, virtualizing (i) the testing of single and combinatorial drug response testing ofon large patient groups, e.g. for clinical trials for drug approval, or for the identification of patient cohorts, including biomarkers, for companion diagnostics, (ii) the testing of combinatory drug response on large patient groups, e.g. for clinical trials for drug approval or for the identification of patient cohorts including biomarkers for companion diagnostics, (iii) the repurposing (repositioning) of drugs from other disease indications for the treatment of cancer and (iiiv) the rescuing of drugs that failed in stage II and III of clinical trials due to because of low efficiency rates rather than non- tolerable side effects for the patients. Such drugs can be approved for particular (responding) patient groups. For virtual clinical trials, we are using the ever-expanding data sets being generated on cancer patients from large, international research projects (e.g. the International Cancer Genome Consortium, ICGC), our own project generated data sets or those dedicated data sets provided by the customers/collaboration partners. We identify, by means of our ‘virtual clinical trial’ system, those patients who would benefit from a specific new or ‘rescued’ drug(s); information that is subsequently exploited to enable approval of the drug in a smaller, more efficient and targeted real-life clinical trial.