NGS services

Certified NGS services

Our customers appreciate the closed, confidential and secure IT infrastructure with ISO9001, Illumina CSPro and Agilent Service Provider certified sequencing pipeline. We are operating all NGS procedures according to strict quality control standards.

Our NGS activities are implemented in a number of research projects and translational activities. We own several patent applications, one of which was licensed by NGS market leaders.

Our NGS service list

  • Whole genome sequencing (WGS)
  • Shallow WGS for CNV analysis
  • Whole exome sequencing (WES)
  • Targeted sequencing
  • Strand-specific RNA sequencing (mRNA and ribo-depleted)
  • Small RNA /microRNA sequencing
  • ChIP-Seq (Chromatin Immuno Precipitation DNA-Sequencing)
  • MeDIP-Seq (Methylated DNA Immuno Precipitation Sequencing)
  • Immune repertoire sequencing

Focus on High Resolution Transcriptomics

Alacris theranostics staff members are inventors of a strand-specific RNAseq protocol (Parkhomchuk et al., Sultan et al.) now used by market leaders in the NGS field. We were first to publish the use of RNAseq comparing to microarrays (Sultan et al.). We know what matters.

Strand-specific high resolution transcriptomics provides an essential data layer for:

  • Identifying cancer driving fusion genes
  • Quantitative gene expression
  • Validating variants indentified by WGS/WES
  • Gene variant allele-specific expression
  • Detecting long non-coding RNAs
  • Identifying differentially spliced transcripts isoforms
  • Identifying novel transcription units
  • Analyzing eRNAs
  • Analyzing eQTLs
  • Identifying specific signatures and biomarkers

NGS-Associated Services

Project planning:

  • Selection of the optimal cost-efficient sequencing startegy
  • Assistance in experimental design

Sample processing:

  • Isolation of nucleic acids from different samples (cell cultures, frozen tissue, blood, FFPE, etc.)
  • DNA and RNA quantification and quality control

Sequencing libraries:

  • Standard and project-specific library preparation
  • Protocols with built-in QC steps
  • Quality check of libraries